Anticorps Polyclonal de lapin anti-GPR177

• Phare
• Validé par KD/KO

GPR177 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 17950-1-AP

Synonymes

C1orf139, EVI, G protein coupled receptor 177, GPR177, MRP, Protein wntless homolog, Sprinter, WLS, Wntless

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Galerie de données de validation

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  WB analysis of HEK-293 using 17950-1-AP

  WB result of GPR177 antibody (17950-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPR177 transfected HEK-293 cells.

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  WB analysis using 17950-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17950-1-AP

  IP result of anti-GPR177 (IP:17950-1-AP, 3ug; Detection:17950-1-AP 1:500) with mouse liver tissue lysate 12500ug.

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu ovarien humain, tissu hépatique de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 4 publications below

Informations sur le produit

17950-1-AP cible GPR177 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR177 Protéine recombinante Ag12428
• Nom complet: G protein-coupled receptor 177
• Masse moléculaire calculée: 541 aa, 62 kDa
• Poids moléculaire observé: 46-65 kDa
• Numéro d’acquisition GenBank: BC110826
• Symbole du gène: GPR177
• Identification du gène (NCBI): 79971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Publications

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
rat	WB	Front Physiol; A Longitudinal Study of T2 Mapping Combined With Diffusion Tensor Imaging to Quantitatively Evaluate Tissue Repair of Rat Skeletal Muscle After Frostbite.; Authors - Yue Gao
human	WB,IHC	Onco Targets Ther; Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer.; Authors - Dandan Zheng
mouse	IHC	Reprod Sci; WNT5A Enhances LH-Mediated Expression of HAS2 in Granulosa Cells.; Authors - Qun Niu
mouse	WB	Int Ophthalmol; The effect of Wnt/β-catenin pathway on the scleral remolding in the mouse during form deprivation.; Authors - Shuyu Hu
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang