Anticorps Polyclonal de lapin anti-gephyrin

gephyrin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (2)

N° de cat : 12681-1-AP

Synonymes

Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase

---

Galerie de données de validation

• 

  WB analysis using 12681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12681-1-AP

  IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of rat brain using 12681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 12681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 12681-1-AP (gephyrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12681-1-AP cible gephyrin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: gephyrin Protéine recombinante Ag3370
• Nom complet: gephyrin
• Masse moléculaire calculée: 769 aa, 83 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC030016
• Symbole du gène: GPHN
• Identification du gène (NCBI): 10243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

Publications

Species	Application	Title
mouse	WB	J Neurosci; Microglial Tmem59 Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice.; Authors - Jian Meng
mouse	WB	Cell Rep; Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.; Authors - Giuseppe Sciamanna
mouse	WB	Neurosci Lett; Microglia participate in postoperative cognitive dysfunction by mediating the loss of inhibitory synapse through the complement pathway; Authors - Xiaoxiang Tan
mouse	WB	Synapse; Triptolide injection reduces Alzheimer's disease-like pathology in mice; Authors - Rui Mao
mouse	IF	Arthritis Rheumatol; Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction; Authors - Zsuzsanna H McMahan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Reyes (Verified Customer) (03-01-2024)

Gephyrin antibody (in red) works on human brain paraffin sections, although being found in the synapsis would make it difficult to quantify in the tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

gephyrin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12681-1-AP)

FH

Deria (Verified Customer) (07-14-2022)

This antibody works fine

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: brain tissue