Anticorps Polyclonal de lapin anti-GPD1L

• Phare
• Validé par KD/KO

GPD1L Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 17263-1-AP

Synonymes

GPD1 L, GPD1L, KIAA0089

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Galerie de données de validation

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  WB analysis of mouse heart using 17263-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17263-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17263-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, tissu cérébral humain, tissu de muscle squelettique humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

17263-1-AP cible GPD1L dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPD1L Protéine recombinante Ag10977
• Nom complet: glycerol-3-phosphate dehydrogenase 1-like
• Masse moléculaire calculée: 351 aa, 38 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC028726
• Symbole du gène: GPD1L
• Identification du gène (NCBI): 23171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.

Publications

Species	Application	Title
human	WB	Cell Metab; Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease.; Authors - Shanshan Liu
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
rat	WB	J Transl Med; Atrial myocyte-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation; Authors - Hongting Hao
human	WB	Cell Death Dis; A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer.; Authors - Ye Du
human,mouse	WB	Mol Cell; Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis; Authors - Cong-Hui Yao ; KD Validated
human	WB	Mol Cell; Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cells; Authors - Yahui Wang