Anticorps Polyclonal de lapin anti-GNMT

GNMT Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 18790-1-AP

Synonymes

glycine N methyltransferase, GNMT

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Galerie de données de validation

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  WB analysis of rat liver using 18790-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human prostate hyperplasia using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules LNCaP, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 24 publications below
• IHC: See 2 publications below

Informations sur le produit

18790-1-AP cible GNMT dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNMT Protéine recombinante Ag4598
• Nom complet: glycine N-methyltransferase
• Masse moléculaire calculée: 295 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC032627
• Symbole du gène: GNMT
• Identification du gène (NCBI): 27232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Publications

Species	Application	Title
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
human	IHC	Int J Mol Sci; MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients.; Authors - Pei-Yi Chu
	WB	J Anim Sci Biotechnol; Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.; Authors - Nagmeldin A Omer
human	WB	Mol Oncol; Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.; Authors - Jie Li
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia