Anticorps Polyclonal de lapin anti-GNL1

GNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14078-1-AP

Synonymes

HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

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Galerie de données de validation

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  WB analysis using 14078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14078-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14078-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14078-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14078-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GNL1 antibody 14078-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNL1 antibody (14078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules Raji, cellules U-937
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14078-1-AP cible GNL1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNL1 Protéine recombinante Ag5203
• Nom complet: guanine nucleotide binding protein-like 1
• Masse moléculaire calculée: 607 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC018366
• Symbole du gène: GNL1
• Identification du gène (NCBI): 2794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide-binding protein-like 1 (GNL1), also known as HSR1, belongs to HSR1_MMR1 subfamily of nucleolar GTPases. It encodes 607 amino acids with a molecular mass of 69 kDa and contains basic amino acids rich N-terminus, acidic amino acids rich C-terminus and proline rich-domains. GNL1 plays a critical role in liver cell proliferation, but its function remains largely unknown (PMID: 18255255).

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu