Anticorps Polyclonal de lapin anti-GNE

GNE Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 25079-1-AP

Synonymes

DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase

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Galerie de données de validation

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  WB analysis using 25079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25079-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25079-1-AP

  IP result of anti-GNE (IP:25079-1-AP, 4ug; Detection:25079-1-AP 1:500) with Jurkat cells lysate 3600ug.

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules Jurkat, tissu hépatique de rat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

25079-1-AP cible GNE dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNE Protéine recombinante Ag18689
• Nom complet: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Masse moléculaire calculée: 722 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC121179
• Symbole du gène: GNE
• Identification du gène (NCBI): 10020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Neoplasma; miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway; Authors - Chun-Ming Li
human	WB	Cell Rep; Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins.; Authors - Joseph Hiatt
mouse	WB,IF,IP	Front Genome Ed; In vivo and in vitro genome editing to explore GNE functions; Authors - Nili Ilouz ; KO Validated
human	IHC	Cells; Loss of GNE Predicts Lymph Node Metastasis in Early Gastric Cancer; Authors - Xinying Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Works well @ 1:50 for IHC on FFPE prostate tissue and 1:2000 for WB on LNCaP cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate