Anticorps Polyclonal de lapin anti-GNB1

• Phare
• Validé par KD/KO

GNB1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10247-2-AP

Synonymes

GNB1, Transducin beta chain 1

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Galerie de données de validation

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  WB analysis using 10247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10247-2-AP (GNB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10247-2-AP (GNB1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10247-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (10247-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Informations sur le produit

10247-2-AP cible GNB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNB1 Protéine recombinante Ag0313
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 1
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC004186
• Symbole du gène: GNB1
• Identification du gène (NCBI): 2782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

Publications

Species	Application	Title
mouse	WB,IF,IP	Cell; Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.; Authors - Gonzalo P Solis ; KD Validated
human	IF,IP	PLoS Pathog; H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans.; Authors - Fangtao Li
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	IHC	Exp Eye Res; Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.; Authors - Haider Neena B NB
mouse	WB	Dev Dyn; Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.; Authors - Okae Hiroaki H
human	WB	Bioengineered; Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer.; Authors - ZhiWei Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nick (Verified Customer) (04-20-2022)

If I could give this three thumbs up, I would.

• Applications: Western Blot