Anticorps Polyclonal de lapin anti-GNAQ

• Phare
• Validé par KD/KO

GNAQ Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13927-1-AP

Synonymes

G ALPHA q, GAQ, GNAQ

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Galerie de données de validation

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  WB analysis using 13927-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13927-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 13927-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13927-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13927-1-AP (GNAQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu cutané de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

13927-1-AP cible GNAQ dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAQ Protéine recombinante Ag4947
• Nom complet: guanine nucleotide binding protein (G protein), q polypeptide
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC057777
• Symbole du gène: GNAQ
• Identification du gène (NCBI): 2776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Publications

Species	Application	Title
mouse	WB	J Exp Med; Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.; Authors - Miwa Sasai
mouse	WB,IF	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
mouse	WB	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li
human	WB	Proteomics; iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.; Authors - Hao Chen
human	WB,IHC	Oncol Rep; G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.; Authors - Yizhuo Wang ; KD Validated
human	WB,IF	J Biol Chem; Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890; Authors - Clinita E Randolph