Anticorps Polyclonal de lapin anti-GNAI3

GNAI3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11641-1-AP

Synonymes

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, 87U6

---

Galerie de données de validation

• 

  WB analysis using 11641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11641-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11641-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11641-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNAI3 antibody (11641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of A431 using 11641-1-AP

  1X10^6 A431 cells were stained with .2ug GNAI3 antibody (11641-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Applications testées

• Résultats positifs en WB: tissu rénal de rat, cellules A431, tissu cérébral de souris, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11641-1-AP cible GNAI3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAI3 Protéine recombinante Ag2229
• Nom complet: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: ~40 kDa
• Numéro d’acquisition GenBank: BC025285
• Symbole du gène: GNAI3
• Identification du gène (NCBI): 2773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

Publications

Species	Application	Title
human	WB	Sci Signal; Growth factor-dependent phosphorylation of Gαi shapes canonical signaling by G protein-coupled receptors; Authors - Suchismita Roy
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu