Anticorps Polyclonal de lapin anti-GNAI2

GNAI2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11136-1-AP

Synonymes

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

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Galerie de données de validation

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  WB analysis using 11136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11136-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 11136-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11136-1-AP

  IP result of anti-GNAI2 (IP:11136-1-AP, 4ug; Detection:11136-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:10 (under 25x lens).

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  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11136-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI2 antibody (11136-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules SKOV-3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu d'amygdalite humain, tissu de cancer du poumon humain, tissu ovarien de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11136-1-AP cible GNAI2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAI2 Protéine recombinante Ag1597
• Nom complet: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC012138
• Symbole du gène: GNAI2
• Identification du gène (NCBI): 2771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
human	WB	Oncotarget; PMID: 24658464; Authors - Jie Li
mouse	IF	bioRxiv; Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis; Authors - Amandine Jarysta
mouse	WB	Adv Sci (Weinh); Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC; Authors - Xinyuan Zhao
human	WB,CoIP	Sci Adv; RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells; Authors - Anil Akturk