Anticorps Monoclonal anti-GMF Beta

GMF Beta Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3B10A2

N° de cat : 60062-1-Ig

Synonymes

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

---

Galerie de données de validation

• 

  WB analysis of fetal human brain using 60062-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 60062-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60062-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 60062-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of U-87 MG using 60062-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60062-1-Ig

  Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu hépatique de porc
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MG U-87, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

60062-1-Ig cible GMF Beta dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GMF Beta Protéine recombinante Ag1104
• Nom complet: glia maturation factor, beta
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC005359
• Symbole du gène: GMFB
• Identification du gène (NCBI): 2764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

Publications

Species	Application	Title
human	IP	Transl Psychiatry; Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein.; Authors - Brawnie Petrov
human	IF	Front Oncol; Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma.; Authors - Tong Zhu
human	IF	J Alzheimers Dis; Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain.; Authors - Mohammad Ejaz Ahmed
human, mouse	IF	PLoS One; Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.; Authors - Duraisamy Kempuraj