Anticorps Polyclonal de lapin anti-GLUD1

• Phare
• Validé par KD/KO

GLUD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

45

N° de cat : 14299-1-AP

Synonymes

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

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Galerie de données de validation

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  WB analysis using 14299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14299-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14299-1-AP

  IP result of anti-GLUD1 (IP:14299-1-AP, 4ug; Detection:14299-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules L02, cellules PC-3, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 41 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14299-1-AP cible GLUD1 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLUD1 Protéine recombinante Ag5694
• Nom complet: glutamate dehydrogenase 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC040132
• Symbole du gène: GLUD1
• Identification du gène (NCBI): 2746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Publications

Species	Application	Title
		Science; Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.; Authors - Jessica B Spinelli
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Cell; Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.; Authors - Wen Yang
human	WB	Cancer Cell; Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.; Authors - Meng Li
mouse	WB	Nat Metab; Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis; Authors - Grace Park
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang