Anticorps Polyclonal de lapin anti-GLRB

GLRB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15371-1-AP

Synonymes

GLRB, Glycine receptor subunit beta, glycine receptor, beta

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Galerie de données de validation

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  WB analysis of mouse brain using 15371-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15371-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spinal cord using 15371-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de moelle épinière de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15371-1-AP cible GLRB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLRB Protéine recombinante Ag4429
• Nom complet: glycine receptor, beta
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC032635
• Symbole du gène: GLRB
• Identification du gène (NCBI): 2743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publications

Species	Application	Title
mouse	WB	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
mouse	WB	Psychopharmacology (Berl); Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.; Authors - Niannian Huang
mouse	WB	J Neurosci; Spinal cord synaptic plasticity by GlyRβ release from receptor fields and syndapin I-dependent uptake; Authors - Jessica Tröger

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

A (Verified Customer) (11-11-2019)

Immunofluorescent analysis of (4% PFA) fixed rat DRG slices using GlyRB rabbit polyclonal antibody at 1:1000 dilution and Alexa 555 at 1:1000 dilution.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat DRG

GLRB Antibody Immunofluorescence, validation (1:1000 dilution) in Rat DRG (Cat no:15371-1-AP)