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Anticorps Polyclonal de lapin anti-GLRA3

GLRA3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 13145-1-AP

Synonymes

GLRA3, glycine receptor, alpha 3

Galerie de données de validation

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  • Western Blot (WB) analysis of MCF-7 cells using GLRA3 Polyclonal antibody (13145-1-AP)

    WB analysis of MCF-7 using 13145-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13145-1-AP (GLRA3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules MCF7

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 2 publications below

Informations sur le produit

13145-1-AP cible GLRA3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GLRA3 Protéine recombinante Ag3702
Nom complet glycine receptor, alpha 3
Masse moléculaire calculée 464 aa, 54 kDa
Poids moléculaire observé 54 kDa
Numéro d’acquisition GenBankBC036086
Symbole du gène GLRA3
Identification du gène (NCBI) 8001
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protocole

Product Specific Protocols
WB protocol for GLRA3 antibody 13145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Biol

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Authors - Zi-Yang Zhang
View Article
humanWB

Neuropharmacology

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Authors - Zi-Yang Zhang
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Braulio (Verified Customer) (02-26-2020)

Western blot in lumbar spinal cord samples, shows a mild band for alpha 3

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Spinal cord