Anticorps Polyclonal de lapin anti-Gastrokine 1

Gastrokine 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19344-1-AP

Synonymes

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

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Galerie de données de validation

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  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 19344-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain, tissu d'estomac de souris
• Résultats positifs en IHC: tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

19344-1-AP cible Gastrokine 1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gastrokine 1 Protéine recombinante Ag6091
• Nom complet: gastrokine 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC059778
• Symbole du gène: Gastrokine 1
• Identification du gène (NCBI): 56287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Gastrokine-1, an anti-amyloidogenic protein secreted by the stomach, regulates diet-induced obesity.; Authors - Anne-Marie C Overstreet
human	WB,IHC	Helicobacter; Helicobacter pylori inhibits GKN1 expression via the CagA/p-ERK/AUF1 pathway.; Authors - Yanlei Guo
mouse	WB,IF,ELISA	Int J Biol Sci; IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1; Authors - Kewei Liu
mouse	WB	Cell Mol Gastroenterol Hepatol; Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia; Authors - Pawan Puri