Anticorps Polyclonal de lapin anti-GIPC1

• Phare
• Validé par KD/KO

GIPC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (2)

N° de cat : 14822-1-AP

Synonymes

C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2

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Galerie de données de validation

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  WB analysis using 14822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14822-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14822-1-AP

  IP result of anti-GIPC1 (IP:14822-1-AP, 4ug; Detection:14822-1-AP 1:500) with mouse brain tissue lysate 9500ug.

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  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14822-1-AP

  Immunofluorescent analysis of HepG2 cells using 14822-1-AP (GIPC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A2780 using 14822-1-AP

  Immunofluorescent analysis of A2780 cells, using 14822-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A2780

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14822-1-AP cible GIPC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GIPC1 Protéine recombinante Ag6597
• Nom complet: GIPC PDZ domain containing family, member 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC000410
• Symbole du gène: GIPC1
• Identification du gène (NCBI): 10755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAIP interacting protein C terminus, member 1(GIPC1), also known as PDZ domain-containing protein GIPC1, is a cytoplasmic protein that also localizes to the peripheral membrane, acting as an adaptor protein linking receptor interactions to intracellular signaling pathways, including cell cycle regulation, and expression has been associated with some cancers (PMID: 19175293, 26757732). This antibody is specific to GIPC1 with a band at 42 kDa (PMID: 18721486).

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.; Authors - Jianwen Deng
human	IHC	Dev Cell; SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.; Authors - Christoph J Burckhardt
human	WB	Ann Neurol; GIPC1 CGG repeat expansion is associated with movement disorders.; Authors - Yu Fan
mouse	WB	Pain; G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.; Authors - Bao-Chun Jiang
mouse	WB	FASEB J; BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.; Authors - Nahoko Eiraku ; KD Validated
human,mouse	WB	CNS Neurosci Ther; G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.; Authors - Yong Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (11-02-2023)

Used at 1:2000 on PVDF membrane, overnight incubation at 4C in 3% BSA PBS 0.1% Tween. No non-specific bands and band reduced by knockdown with GIPC1 siRNA. Diluted antibody succesfully re0used after freezing at -20C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HUVEC

GIPC1 Antibody Western Blot validation (1:2000 dilution) in HUVEC (Cat no:14822-1-AP)

FH

Sophy (Verified Customer) (01-27-2023)

I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells