Anticorps Polyclonal de lapin anti-GGCX

• Phare
• Validé par KD/KO

GGCX Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16209-1-AP

Synonymes

gamma glutamyl carboxylase, GC, GGCX, VKCFD1

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Galerie de données de validation

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  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16209-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16209-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16209-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16209-1-AP

  IP result of anti-GGCX (IP:16209-1-AP, 4ug; Detection:16209-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules HepG2, cellules L02, cellules MCF-7, cellules PC-3, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

16209-1-AP cible GGCX dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGCX Protéine recombinante Ag9185
• Nom complet: gamma-glutamyl carboxylase
• Masse moléculaire calculée: 758 aa, 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC013979
• Symbole du gène: GGCX
• Identification du gène (NCBI): 2677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

Publications

Species	Application	Title
mouse	WB	J Cell Biol; GGCX and VKORC1 inhibit osteocalcin endocrine functions.; Authors - Mathieu Ferron
		Blood; Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders.; Authors - Jian-Ke Tie
human	WB	Methods Enzymol; Functional Study of the Vitamin K Cycle Enzymes in Live Cells.; Authors - J-K Tie ; KO Validated
human	WB	JCI Insight; VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.; Authors - Julie Lacombe
human	WB	Oncotarget; Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells.; Authors - Sarah Beaudin
human,mouse	WB,IP	Cell Rep; Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells; Authors - Julie Lacombe