Anticorps Polyclonal de lapin anti-GGCT

GGCT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16257-1-AP

Synonymes

C7orf24, CRF21, Gamma glutamylcyclotransferase, GCTG, Ggc, GGCT

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Galerie de données de validation

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  WB analysis using 16257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16257-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 16257-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16257-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16257-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16257-1-AP

  IP result of anti-GGCT (IP:16257-1-AP, 4ug; Detection:16257-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

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  IHC staining of mouse small intestine using 16257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 16257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16257-1-AP (GGCT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules U-937, tissu cérébral humain, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu d'intestin grêle de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

16257-1-AP cible GGCT dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGCT Protéine recombinante Ag9284
• Nom complet: gamma-glutamyl cyclotransferase
• Masse moléculaire calculée: 188 aa, 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC019243
• Symbole du gène: GGCT
• Identification du gène (NCBI): 79017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean
human	WB	Sci Rep; Identification of proteins responsible for adriamycin resistance in breast cancer cells using proteomics analysis.; Authors - Zhipeng Wang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages; Authors - Lin Shen
human	WB,IHC,CoIP	Int J Mol Sci; Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer; Authors - Hui-Min Zhang
mouse	WB,IHC	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano
mouse	WB,IHC	Cancer Sci; Myc upregulates Ggct, γ-glutamylcyclotransferase to promote development of p53-deficient osteosarcoma; Authors - Tomoya Ueno