Anticorps Polyclonal de lapin anti-GFPT1

• Phare
• Validé par KD/KO

GFPT1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 14132-1-AP

Synonymes

GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 14132-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14132-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14132-1-AP

  Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, cellules HepG2
• Résultats positifs en IHC: tissu de gliome humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14132-1-AP cible GFPT1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFPT1 Protéine recombinante Ag5312
• Nom complet: glutamine-fructose-6-phosphate transaminase 1
• Masse moléculaire calculée: 681 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC045641
• Symbole du gène: GFPT1
• Identification du gène (NCBI): 2673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
		Circ Res; Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.; Authors - Felipe Paredes
human	IF	J Clin Invest; Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.; Authors - Nikita S Sharma
	WB	Nat Commun; Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.; Authors - Adela S Oliva Chávez
human	WB	Am J Hum Genet; Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.; Authors - Senderek Jan J

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

Mouse testis were stained for Gfpt1 (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse testis

GFPT1 Antibody Immunofluorescence validation (1:200 dilution) in Mouse testis (Cat no:14132-1-AP)