Anticorps Recombinant de lapin anti-GFAP

• Phare
• Validé par KD/KO

GFAP Recombinant Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

4C6

Avis (1)

N° de cat : 81063-1-RR

Synonymes

4C6, glial fibrillary acidic protein

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Galerie de données de validation

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  WB analysis of U-251 using 81063-1-RR

  U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 81063-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 81063-1-RR

  WB result of GFAP antibody (81063-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

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  IHC staining of rat brain using 81063-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 81063-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 81063-1-RR

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 81063-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 81063-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 81063-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of rat brain using 81063-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, green).

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  Affinity and Kinetic Characterization of 81063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules U-251, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF-Fro: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:4950-1:19800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81063-1-RR cible GFAP dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GFAP Protéine recombinante Ag10423
• Nom complet: glial fibrillary acidic protein
• Masse moléculaire calculée: 432 aa, 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC013596
• Symbole du gène: GFAP
• Identification du gène (NCBI): 2670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kateryna (Verified Customer) (01-23-2025)

We use the GFAP antibody to visualize astrocytes via immunostaining in mouse brain tissue. The mouse brain tissue is fixed, brain sections are 40 μm thick, and blocked with 3% normal goat serum. The incubation with the primary antibody (GFAP) is performed at 4°C overnight, followed by the corresponding Alexa 633-labeled anti-rabbit fluorescent secondary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain tissue

GFAP Antibody Immunofluorescence validation (1:100 dilution) in Mouse brain tissue (Cat no:81063-1-RR)

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