Anticorps Polyclonal de lapin anti-GEFT-Specific

GEFT-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 14839-1-AP

Synonymes

ARHGEF25, GEFT, GEFT-Specific, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14839-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14839-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14839-1-AP

  IP result of anti-GEFT-Specific (IP:14839-1-AP, 4ug; Detection:14839-1-AP 1:1500) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human skeletal muscle using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human skeletal muscle using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HEK-293, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

14839-1-AP cible GEFT-Specific dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RhoA/RAC/CDC42 exchange factor
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: NM_182947
• Symbole du gène: GEFT
• Identification du gène (NCBI): 115557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

Publications

Species	Application	Title
mouse	WB	Genes Dev; TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway.; Authors - Kant Shashi S
human	WB	Circ Res; p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.; Authors - Momotani Ko K
mouse	WB	Sci Signal; RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger.; Authors - Mykhaylo V Artamonov
rat	WB,IHC	FASEB J; p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.; Authors - Wuertz Christina M CM
mouse	WB,IHC,IF	Mol Cell Biol; Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.; Authors - Bryan Brad A BA
human	WB	J Hypertens; Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients.; Authors - Lorenzo A Calò

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joelle (Verified Customer) (09-10-2019)

Overnight at 4 degrees

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HHL16

GEFT-Specific Antibody Western Blot, validation (1/1000 dilution) in HHL16 (Cat no:14839-1-AP)