Anticorps Polyclonal de lapin anti-GCLM

• Phare
• Validé par KD/KO

GCLM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

131

Avis (1)

N° de cat : 14241-1-AP

Synonymes

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

---

Galerie de données de validation

• 

  WB analysis using 14241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14241-1-AP

  WB result of GCLM antibody (14241-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.

• 

  WB analysis using 14241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 14241-1-AP

  IP result of anti-GCLM (IP:14241-1-AP, 4ug; Detection:14241-1-AP 1:2000) with A431 cells lysate 1000 ug.

• 

  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14241-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GCLM antibody (14241-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 14241-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GCLM (14241-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 123 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Informations sur le produit

14241-1-AP cible GCLM dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCLM Protéine recombinante Ag5497
• Nom complet: glutamate-cysteine ligase, modifier subunit
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC041809
• Symbole du gène: GCLM
• Identification du gène (NCBI): 2730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Publications

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
mouse	WB	Sci Adv; Immunomodulatory actions of a kynurenine-derived endogenous electrophile.; Authors - Mara Carreño
mouse	WB	Nat Commun; Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.; Authors - Yao Wei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (01-17-2023)

Good antibody for WB. There are some non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hela, human liver and mouse liver

GCLM Antibody Western Blot validation (1:2000 dilution) in Hela, human liver and mouse liver (Cat no:14241-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C