Anticorps Polyclonal de lapin anti-GCLC

• Phare
• Validé par KD/KO

GCLC Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

113

Avis (1)

N° de cat : 12601-1-AP

Synonymes

Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC

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Galerie de données de validation

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  WB analysis using 12601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12601-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12601-1-AP

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.

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  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules L02, cellules Raji, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 111 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

12601-1-AP cible GCLC dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCLC Protéine recombinante Ag3252
• Nom complet: glutamate-cysteine ligase, catalytic subunit
• Masse moléculaire calculée: 637 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC022487
• Symbole du gène: GCLC
• Identification du gène (NCBI): 2729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang ; KO Validated
human	WB	Cell; Differential Requirements for eIF4E Dose in Normal Development and Cancer.; Authors - Morgan L Truitt
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

AIK SENG (Verified Customer) (01-10-2023)

Clear & crisp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Colorectal cancer