Anticorps Polyclonal de lapin anti-GCK

• Phare
• Validé par KD/KO

GCK Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15629-1-AP

Synonymes

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2

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Galerie de données de validation

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  WB analysis using 15629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15629-1-AP

  IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.

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  IHC staining of human liver cancer using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

15629-1-AP cible GCK dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCK Protéine recombinante Ag7904
• Nom complet: glucokinase (hexokinase 4)
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC001890
• Symbole du gène: GCK
• Identification du gène (NCBI): 2645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human.

Publications

Species	Application	Title
mouse	WB	J Diabetes Investig; Using recombinase-mediated cassette exchange to engineer MIN6 insulin-secreting cells based on a newly identified safe harbor locus.; Authors - Asami Furukawa ; KD Validated
mouse	WB	Mol Cell Endocrinol; Improved lipogenesis gene expression in liver is associated with elevated plasma angiotensin 1-7 after AT1 receptor blockade in insulin-resistant OLETF rats; Authors - Jose A Godoy-Lugo