Anticorps Polyclonal de lapin anti-GBE1

• Phare
• Validé par KD/KO

GBE1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 20313-1-AP

Synonymes

Brancher enzyme, GBE, GBE1, Glycogen branching enzyme

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Galerie de données de validation

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  WB analysis using 20313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20313-1-AP (GBE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20313-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20313-1-AP (GBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20313-1-AP

  IP result of anti-GBE1 (IP:20313-1-AP, 4ug; Detection:20313-1-AP 1:2000) with L02 cells lysate 1360 ug.

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  IF Staining of HeLa using 20313-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBE1 antibody (20313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu de muscle squelettique humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below

Informations sur le produit

20313-1-AP cible GBE1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GBE1 Protéine recombinante Ag14154
• Nom complet: glucan (1,4-alpha-), branching enzyme 1
• Masse moléculaire calculée: 702 aa, 80 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC012098
• Symbole du gène: GBE1
• Identification du gène (NCBI): 2632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GBE1 (glucan (1,4-alpha-), branching enzyme 1) is essential for the globular and branched structure of glycogen, increasing solubility by creating a hydrophilic surface and reducing intracellular osmotic pressure. It may serve as a potential therapeutic target for treating LUAD(PMID: 31221150). The full sequence can be further processed into a mature form of 75 kDa.

Publications

Species	Application	Title
human	WB	Am J Hum Genet; Loss of C2orf69 defines a fatal autoinflammatory syndrome in humans and zebrafish that evokes a glycogen storage-associated mitochondriopathy.; Authors - Hui Hui Wong ; KD Validated
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
mouse	WB	J Proteome Res; Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.; Authors - Jingzi Zhang
rat	WB	PLoS One; A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise; Authors - Seong Eun Kwak