Anticorps Polyclonal de lapin anti-GAS6

GAS6 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13795-1-AP

Synonymes

AXLLG, AXSF, GAS 6, GAS6, growth arrest 6

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Galerie de données de validation

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  WB analysis of human urine using 13795-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13795-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13795-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13795-1-AP (GAS6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: échantillon d'urine humaine, cellules DU 145, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

13795-1-AP cible GAS6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAS6 Protéine recombinante Ag4617
• Nom complet: growth arrest-specific 6
• Masse moléculaire calculée: 678 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC038984
• Symbole du gène: GAS6
• Identification du gène (NCBI): 2621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Growth arrest-specific protein 6 (GAS6) is also named as AXLLG. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses (PMID:12364394, PMID:18840707). It can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PMID:21501828).

Publications

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	WB	FASEB J; Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases.; Authors - Hua Qian
human	WB,IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	Transplantation; Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.; Authors - Zhen Wang
human	WB	Mol Immunol; The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury; Authors - Yujing Cheng