Anticorps Polyclonal de lapin anti-GANP

• Phare
• Validé par KD/KO

GANP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11054-1-AP

Synonymes

MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48

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Galerie de données de validation

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  WB analysis of mouse brain using 11054-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11054-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11054-1-AP

  IP result of anti-GANP (IP:11054-1-AP, 4ug; Detection:11054-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11054-1-AP (GANP antibody) at dilution of 1:10 (under 10x lens).

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  IF Staining of HepG2 using 11054-1-AP

  Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody 11054-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 11054-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GANP Polyclonal antibody (11054-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules NIH/3T3
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

11054-1-AP cible GANP dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GANP Protéine recombinante Ag1486
• Nom complet: minichromosome maintenance complex component 3 associated protein
• Masse moléculaire calculée: 218 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC013285
• Symbole du gène: GANP
• Identification du gène (NCBI): 8888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.

Publications

Species	Application	Title
human	WB	Nat Commun; GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.; Authors - Singh Shailendra Kumar SK ; KD Validated
human	WB	J Immunol; GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.; Authors - Maeda Kazuhiko K ; KD Validated
human	WB	Front Mol Neurosci; The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.; Authors - Chunqing Yang ; KD Validated
human,monkey	WB,IF	J Biol Chem; GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA.; Authors - Maeda Kazuhiko K