Anticorps Polyclonal de lapin anti-GALNT14

• Phare
• Validé par KD/KO

GALNT14 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16939-1-AP

Synonymes

GalNac T10, GalNac T14, GALNT14, GALNT15, pp GaNTase 14

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 16939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16939-1-AP

  WB result of GALNT14 antibody (16939-1-AP; 1:1200; room temperature for 1.5 hours) with wild-type and GALNT14 knockout A549 cells.

• 

  WB analysis of mouse kidney using 16939-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16939-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 16939-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 Antibody) at dilution of 1:400 incubated at 4 degree celsius over night.

• 

  IHC staining of human kidney using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules A549, tissu pulmonaire de souris, tissu rénal de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

16939-1-AP cible GALNT14 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GALNT14 Protéine recombinante Ag10470
• Nom complet: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14)
• Masse moléculaire calculée: 552 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC010659
• Symbole du gène: GALNT14
• Identification du gène (NCBI): 79623
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Oncotarget; GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma.; Authors - Ok-Seon Kwon ; KD Validated
		Mol Cancer Res; BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14.; Authors - Joanna C Hillman
human	IHC	Front Immunol; Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma; Authors - Jintao Wu
human	WB	Front Immunol; Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma; Authors - Zhongyue Liu ; KD Validated