Anticorps Polyclonal de lapin anti-GALC

GALC Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12887-1-AP

Synonymes

Galactocerebrosidase, galactosylceramidase, GALC, GALCERase

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Galerie de données de validation

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  WB analysis of A375 using 12887-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12887-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12887-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12887-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, tissu cérébral de souris, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

12887-1-AP cible GALC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GALC Protéine recombinante Ag3915
• Nom complet: galactosylceramidase
• Masse moléculaire calculée: 668 aa, 76 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC036518
• Symbole du gène: GALC
• Identification du gène (NCBI): 2581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galactosylceramidase (Galc) is a lysosomal enzyme involved in the catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelial cells of the small intestine and colon. The normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. Mutation of GALC gene will lead to Krabbe disease (globoid cell leukodystrophy), an autosomal recessive neurodegenerative disorder that affects both the central and peripheral nervous system due to an enzymatic defect of galactocerebrosidase.

Publications

Species	Application	Title
human	IHC	Chembiochem; A specific activity-based probe to monitor family GH59 galactosylceramidase - the enzyme deficient in Krabbe disease.; Authors - André Ra Marques