Anticorps Polyclonal de lapin anti-GADD45GIP1

GADD45GIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

5

N° de cat : 16260-1-AP

Synonymes

CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6

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Galerie de données de validation

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  WB analysis using 16260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16260-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16260-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 16260-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16260-1-AP

  IP result of anti-GADD45GIP1 (IP:16260-1-AP, 4ug; Detection:16260-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules NIH/3T3, cellules SKOV-3, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16260-1-AP cible GADD45GIP1 dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GADD45GIP1 Protéine recombinante Ag9299
• Nom complet: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC013027
• Symbole du gène: GADD45GIP1
• Identification du gène (NCBI): 90480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Science; T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.; Authors - Isobel Okoye
human	IHC	Oncotarget; CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.; Authors - Runzhou Zhuang
rat	WB	J Mol Med (Berl); Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.; Authors - Li Peng
mouse	WB	Burns Trauma; Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.; Authors - Lingfei Li
mouse	WB,IHC	Int J Mol Sci; Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1; Authors - Kazuhiro Hasegawa