Anticorps Polyclonal de lapin anti-GAD65

GAD65 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21760-1-AP

Synonymes

GAD2, GAD-65, GAD 65, EC:4.1.1.15

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Galerie de données de validation

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  WB analysis using 21760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21760-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21760-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 21760-1-AP

  IP result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 21760-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21760-1-AP (GAD65 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 21760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21760-1-AP (GAD65 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21760-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAD65 antibody (21760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:100-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

21760-1-AP cible GAD65 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAD65 Protéine recombinante Ag16251
• Nom complet: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Masse moléculaire calculée: 585 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC126327
• Symbole du gène: GAD65
• Identification du gène (NCBI): 2572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Publications

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
mouse	WB	J Neuroinflammation; FKBP51 mediates resilience to inflammation-induced anxiety through regulation of glutamic acid decarboxylase 65 expression in mouse hippocampus.; Authors - Yu-Ling Gan
human	WB	Neuropharmacology; Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.; Authors - Yue Li
mouse	WB	Neurosci Lett; Repeated maternal separation prolongs the critical period of ocular dominance plasticity in mouse visual cortex.; Authors - Sitong Li