Anticorps Polyclonal de lapin anti-GAA

GAA Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 14367-1-AP

Synonymes

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase

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Galerie de données de validation

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  WB analysis using 14367-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14367-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14367-1-AP

  IP result of anti-GAA (IP:14367-1-AP, 4ug; Detection:14367-1-AP 1:600) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14367-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14367-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules DU 145, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de muscle squelettique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14367-1-AP cible GAA dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAA Protéine recombinante Ag5690
• Nom complet: glucosidase, alpha; acid
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 70-76 kDa
• Numéro d’acquisition GenBank: BC040431
• Symbole du gène: GAA
• Identification du gène (NCBI): 2548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.

Publications

Species	Application	Title
human	IF	Nat Commun; Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis; Authors - Lindsey R Conroy
human	IF	J Med Chem; 5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease.; Authors - Atsushi Kato
human	IF	Org Biomol Chem; Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation; Authors - Atsushi Kato
zebrafish	WB	DNA Cell Biol; Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease.; Authors - Jing Wu
	IF	J Med Chem; Design and Pharmacological Chaperone Effects of N-(4'-Phenylbutyl)-DAB Derivatives Targeting the Lipophilic Pocket of Lysosomal Acid α-Glucosidase; Authors - Atsushi Kato
mouse,rat	WB,IHC	Phytother Res; D-pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin-mediated endoplasmic reticulum stress and glycophagy signaling pathway; Authors - Xiaoli Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHRISTINE (Verified Customer) (09-27-2019)

will continue to order here in the future.

• Applications: Western Blot, Flow Cytometry, Cell culture,
• Cell Tissue Type: IgG