Anticorps Polyclonal de lapin anti-G3BP2

• Phare
• Validé par KD/KO

G3BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 16276-1-AP

Synonymes

G3BP 2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2, G3BP-2

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Galerie de données de validation

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  WB analysis using 16276-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of T47D cells using 16276-1-AP

  WB result of G3BP2 antibody (16276-1-AP, 1:500) with T47D breast cancer cells.

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  WB analysis of MCF-7 using 16276-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16276-1-AP

  IP result of anti-G3BP2 (IP:16276-1-AP, 4ug; Detection:16276-1-AP 1:1000) with HeLa cells lysate 2200 ug.

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  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 16276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16276-1-AP (G3BP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 16276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (16276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules Neuro-2a, cellules T47D
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu hépatique de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: sodium arsenite treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

16276-1-AP cible G3BP2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: G3BP2 Protéine recombinante Ag9355
• Nom complet: GTPase activating protein (SH3 domain) binding protein 2
• Masse moléculaire calculée: 482aa,54 kDa; 449aa,51 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC011731
• Symbole du gène: G3BP2
• Identification du gène (NCBI): 9908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Publications

Species	Application	Title
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human		Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang
human	WB,IP	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated
human	WB,IF,IP	Oncogene; RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.; Authors - Yaqi Chen ; KD Validated
human	WB,IHC	Mol Cancer; Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.; Authors - Hongwei Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Karine (Verified Customer) (09-22-2022)

Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mMNa2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The proteinconcentration was measured with the DC Protein Assay (BIO-RAD) and 30μgof total proteins were loaded onto 12% SDS- polyacrylamide gels forelectrophoresis and transferred onto polyvinylidene difluoride membranes(Millipore). After 1h of blocking with 5% bovine serum albumin preparedin Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots wereincubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhancedchemiluminescence detection kit (Millipore) and and the Syngene Pxi4imaging system (Ozyme).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: H69 cells

G3BP2 Antibody Western Blot validation (1/1000 dilution) in H69 cells (Cat no:16276-1-AP)