Anticorps Polyclonal de lapin anti-G-CSF

• Phare
• Validé par KD/KO

G-CSF Polyclonal Antibody for WB, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

2

N° de cat : 17185-1-AP

Synonymes

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

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Galerie de données de validation

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  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 17185-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human G-CSF (17185-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, cellules MOLT-4
• Résultats positifs en FC (Intra): cellules K-562
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

17185-1-AP cible G-CSF dans les applications de WB, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: G-CSF Protéine recombinante Ag10968
• Nom complet: colony stimulating factor 3 (granulocyte)
• Masse moléculaire calculée: 200 aa, 22 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC033245
• Symbole du gène: G-CSF
• Identification du gène (NCBI): 1440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

Publications

Species	Application	Title
human	IHC	Mol Immunol; Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.; Authors - Zheng Zhang
human	WB,IHC	Int J Mol Sci; A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer; Authors - Wei Jiang ; KD Validated