Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

APC Fluorescent Dye

Publications

3

CloneNo.

3G3

N° de cat : APC-65089

Synonymes

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using APC-65089

  1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65089 cible Foxp3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	FC (Intra)	Drug Deliv; Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.; Authors - Dingyi Wang
mouse	FC (Intra)	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC (Intra)	ACS Nano; Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity; Authors - Yunkai Shang

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011