Anticorps Polyclonal de lapin anti-Fibrinogen Alpha Chain

• Phare
• Validé par KD/KO

Fibrinogen Alpha Chain Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 20645-1-AP

Synonymes

FGA, Fib2, fibrinogen alpha chain

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Galerie de données de validation

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  WB analysis of mouse liver using 20645-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20645-1-AP (Fibrinogen alpha chain antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

20645-1-AP cible Fibrinogen Alpha Chain dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fibrinogen Alpha Chain Protéine recombinante Ag9567
• Nom complet: fibrinogen alpha chain
• Masse moléculaire calculée: 866 aa, 95 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC020764
• Symbole du gène: Fibrinogen Alpha Chain
• Identification du gène (NCBI): 2243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.; Authors - Di-Wei Zheng
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Aging (Albany NY); Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.; Authors - Wenjing Wang
mouse	WB	J Cell Mol Med; Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice; Authors - Guillaume Postic
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	IF	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang