Anticorps Polyclonal de lapin anti-Frizzled 10

Frizzled 10 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 18175-1-AP

Synonymes

CD350, Frizzled 10, FZ 10, FZD10, FzE7, hFz10

---

Galerie de données de validation

• 

  WB analysis of mouse kidney using 18175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

18175-1-AP cible Frizzled 10 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 10 Protéine recombinante Ag12867
• Nom complet: frizzled homolog 10 (Drosophila)
• Masse moléculaire calculée: 581 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC074998
• Symbole du gène: Frizzled 10
• Identification du gène (NCBI): 11211
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.

Publications

Species	Application	Title
mouse	WB,FC	Nat Commun; Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.; Authors - Alison E Jarmas
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
zebrafish	WB	Development; Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.; Authors - Alissa Caron
human	WB	Oncotarget; Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.; Authors - Benjamin A Farber
human	WB	Int J Oncol; Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.; Authors - Qian Sun
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Susanne (Verified Customer) (04-17-2023)

staining was not so nice like Frizzled 5, 7 or 9 from Proteintech

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Fibroblasts

Frizzled 10 Antibody Immunofluorescence validation (1:50 dilution) in Fibroblasts (Cat no:18175-1-AP)

FH

Sylvain (Verified Customer) (12-11-2018)

Perfect signal!

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human colon cancer