Anticorps Polyclonal de lapin anti-FXYD2

FXYD2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

6

N° de cat : 11198-1-AP

Synonymes

Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C

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Galerie de données de validation

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  WB analysis of human kidney using 11198-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11198-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11198-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11198-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11198-1-AP (FXYD2 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, tissu de muscle squelettique humain, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11198-1-AP cible FXYD2 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FXYD2 Protéine recombinante Ag1676
• Nom complet: FXYD domain containing ion transport regulator 2
• Masse moléculaire calculée: 7 kDa
• Poids moléculaire observé: 7-10 kDa
• Numéro d’acquisition GenBank: BC013289
• Symbole du gène: FXYD2
• Identification du gène (NCBI): 486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Publications

Species	Application	Title
mouse	IHC	Cell; Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair.; Authors - Lu Catherine P CP
mouse	IHC	Front Cell Dev Biol; A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.; Authors - Marta Marchini
rat	IHC	Nutrients; Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome.; Authors - Hwee-Yeong Ng
mouse	IF	Cell Rep; Tumor vessel co-option probed by single-cell analysis.; Authors - Laure-Anne Teuwen
human	WB,IF	J Immunol Res; Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma; Authors - Zedan Zhang
mouse	WB	Brain Res Bull; Microglial SIX2 suppresses lipopolysaccharide (LPS)-induced neuroinflammation by up-regulating FXYD2 expression; Authors - Xia-Yin Cao