Anticorps Polyclonal de lapin anti-FXR2

• Phare
• Validé par KD/KO

FXR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12552-1-AP

Synonymes

RNA-binding protein FXR2, FXR2P, FMR1L2, FMR1 autosomal homolog 2

---

Galerie de données de validation

• 

  WB analysis using 12552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12552-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12552-1-AP

  IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

12552-1-AP cible FXR2 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FXR2 Protéine recombinante Ag3402
• Nom complet: fragile X mental retardation, autosomal homolog 2
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC020090
• Symbole du gène: FXR2
• Identification du gène (NCBI): 9513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FXR2 (FMR1 autosomal homolog 2), also known as: FXR2P; Fxr2h, is belong to the Fragile X gene family. FXR2 is a mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis. Also, FXR2 acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus(Uniprot).

Publications

Species	Application	Title
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez
human	WB	Proc Natl Acad Sci U S A; ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation; Authors - Gui-Feng Zhou ; KD Validated