Anticorps Polyclonal de lapin anti-FXR1

• Phare
• Validé par KD/KO

FXR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 13194-1-AP

Synonymes

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1

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Galerie de données de validation

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  WB analysis using 13194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13194-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13194-1-AP

  IP result of anti-FXR1 (IP:13194-1-AP, 3ug; Detection:13194-1-AP 1:800) with mouse skeletal muscle tissue lysate 8000ug.

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13194-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (13194-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13194-1-AP

  Immunofluorescent analysis of HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules NIH/3T3, tissu cardiaque de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• RIP: See 2 publications below

Informations sur le produit

13194-1-AP cible FXR1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FXR1 Protéine recombinante Ag3873
• Nom complet: fragile X mental retardation, autosomal homolog 1
• Masse moléculaire calculée: 621 aa, 70 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC028983
• Symbole du gène: FXR1
• Identification du gène (NCBI): 8087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Publications

Species	Application	Title
human	IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
mouse	IF	Neuron; Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex.; Authors - Gordon X Wang
mouse	IF	Nat Commun; Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle; Authors - Lance T. Denes
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang
		Elife; Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses.; Authors - Gordon X Wang
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chao (Verified Customer) (01-30-2020)

Western blot show strong band in the basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart