Anticorps Polyclonal de lapin anti-FXN

• Phare
• Validé par KD/KO

FXN Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (3)

N° de cat : 14147-1-AP

Synonymes

FA, Extramitochondrial frataxin, EC:1.16.3.1, dFXN, d FXN

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Galerie de données de validation

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  WB analysis using 14147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14147-1-AP (FXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules Jurkat, cellules K-562, cellules Raji, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14147-1-AP cible FXN dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FXN Protéine recombinante Ag5316
• Nom complet: frataxin
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC048097
• Symbole du gène: FXN
• Identification du gène (NCBI): 2395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FXN, also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)

Publications

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	WB	Brain; Autosomal recessive cerebellar ataxia caused by a homozygous mutation in PMPCA.; Authors - Karine Choquet
mouse	WB	Neurobiol Dis; Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin; Authors - Daniel Fil
mouse	WB	J Nutr Biochem; Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction; Authors - Jingjing Liu
human	WB,IHC	Sci Rep; Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia.; Authors - Joseph F Nabhan
human	WB	Hum Mol Genet; Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.; Authors - Yanjie Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: IPSC cardiomyocytes

FH

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 mouse myoblast

FH

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain