Anticorps Polyclonal de lapin anti-FUT4

FUT4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 22141-1-AP

Synonymes

Alpha (1,3) fucosyltransferase, ELFT, FCT3A, FUC TIV, Fucosyltransferase 4, Fucosyltransferase IV, FucT IV, FUT4, FUTIV

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Galerie de données de validation

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  WB analysis of Jurkat using 22141-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22141-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 22141-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FUT4 antibody (22141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22141-1-AP cible FUT4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUT4 Protéine recombinante Ag17565
• Nom complet: fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Masse moléculaire calculée: 530 aa, 59 kDa
• Poids moléculaire observé: 45 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC136374
• Symbole du gène: FUT4
• Identification du gène (NCBI): 2526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 22141-1-AP detects the band around 45 kDa and glycosylated isoform 63 kDa protein in SDS-PAGE. (PMID: 22287018, 17335083, 11278338)