Anticorps Polyclonal de lapin anti-FUT4

• Phare
• Validé par KD/KO

FUT4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

24

N° de cat : 19497-1-AP

Synonymes

FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, Alpha-(1,3)-fucosyltransferase 4

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Galerie de données de validation

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  WB analysis using 19497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19497-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19497-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19497-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19497-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19497-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19497-1-AP (FUT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19497-1-AP

  IP result of anti-FUT4 (IP:19497-1-AP, 4ug; Detection:19497-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human lung cancer using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 19497-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19497-1-AP (FUT4 Antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of A375 using 19497-1-AP

  1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human FUT4 (19497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules SGC-7901
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de la peau humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A375,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 18 publications below
• IHC: See 7 publications below
• IF: See 10 publications below

Informations sur le produit

19497-1-AP cible FUT4 dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 95-140 kDa
• Numéro d’acquisition GenBank: NM_002033
• Symbole du gène: FUT4
• Identification du gène (NCBI): 2526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 19497-1-AP antibody detects the glycosylated isoform proteins around 95-140 kDa in SDS-PAGE. (PMID: 28706275, 28914881, 11278338)

Publications

Species	Application	Title
mouse	IF	ACS Appl Mater Interfaces; Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair.; Authors - Lei Fan
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human,mouse	WB,IF	Cell Death Differ; MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation.; Authors - Qin Zheng ; KD Validated
human	WB,IF	Oncotarget; Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4.; Authors - Lili Tian ; KD Validated
human	WB	Cell Death Dis; Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells.; Authors - Yang X X ; KD Validated
human	IF	Front Mol Neurosci; Transcriptional Repression of p53 by PAX3 Contributes to Gliomagenesis and Differentiation of Glioma Stem Cells.; Authors - Hui Zhu