Anticorps Polyclonal de lapin anti-FUSIP1

• Phare
• Validé par KD/KO

FUSIP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10131-1-AP

Synonymes

40 kDa SR repressor protein, FUSIP1, FUSIP2, NSSR, SFRS13, SFRS13A, Splicing factor SRp38, SRp38, SRrp40, SRSF10, TASR, TASR1, TASR2, TLS associated SR protein

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Galerie de données de validation

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  WB analysis of HepG2 using 10131-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10131-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10131-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human urothelial carcinoma using 10131-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10131-1-AP (FUSIP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10131-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10131-1-AP (FUSIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IHC: tissu de carcinome urothélial humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

10131-1-AP cible FUSIP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUSIP1 Protéine recombinante Ag0176
• Nom complet: FUS interacting protein (serine/arginine-rich) 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC001107
• Symbole du gène: FUSIP1
• Identification du gène (NCBI): 10772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUSIP1, also named as SRrp40, SRp38 ,FUSIP2, SFRS13A, or TASR, is a 262 amino acid protein, which belongs to the splicing factor SR family. FUSIP1 as a Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 is required for splicing repression in M-phase cells and after heat shock and also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. FUSIP1 Interacting with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	J Exp Clin Cancer Res; SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway.; Authors - Xiaobai Liu ; KD Validated
human	WB,IHC	Proteomics Clin Appl; Integration of the cancer cell secretome and transcriptome reveals potential noninvasive diagnostic markers for bladder cancer; Authors - Yi-Ting Chen