Anticorps Polyclonal de lapin anti-FURIN

• Phare
• Validé par KD/KO

FURIN Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (4)

N° de cat : 18413-1-AP

Synonymes

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

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Galerie de données de validation

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  WB analysis of HepG2 using 18413-1-AP

  WB result of FURIN antibody (18413-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FURIN transfected HepG2 cells.

• 

  WB analysis of COLO 320 using 18413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 18413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 18413-1-AP

  IP result of anti-FURIN (IP:18413-1-AP, 4ug; Detection:18413-1-AP 1:4000) with HeLa cells lysate 2000 ug.

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  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

18413-1-AP cible FURIN dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FURIN Protéine recombinante Ag13261
• Nom complet: furin (paired basic amino acid cleaving enzyme)
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC012181
• Symbole du gène: FURIN
• Identification du gène (NCBI): 5045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).

Publications

Species	Application	Title
human	IHC	Cell Rep; Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury; Authors - Yiheng Mao
human	WB	Oncogene; Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.; Authors - Diana Farhat
human	WB	PLoS Pathog; Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2; Authors - Shi Yu ; KD Validated
human	WB	Oncogenesis; Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.; Authors - Huizhi Wang
human	WB	Br J Cancer; Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.; Authors - Diana Farhat ; KD Validated
human	WB,IF	J Virol; Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry; Authors - Timothy J Smith ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (05-31-2024)

WB at 1:1000. Two (80 and 104 kDa) bands detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Wojciech (Verified Customer) (05-21-2024)

Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

FH

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: 293T, HeLa