Anticorps Polyclonal de lapin anti-FOXP3

• Phare
• Validé par KD/KO

FOXP3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 22173-1-AP

Synonymes

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

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Galerie de données de validation

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  WB analysis of HEK-293 using 22173-1-AP

  WB result of FOXP3 antibody (22173-1-AP, 1:500) with si-Control and si-FOXP3 transfected HEK293 cells.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 22173-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

22173-1-AP cible FOXP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXP3 Protéine recombinante Ag17827
• Nom complet: forkhead box P3
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC113401
• Symbole du gène: FOXP3
• Identification du gène (NCBI): 50943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publications

Species	Application	Title
mouse	WB	Exp Ther Med; Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway.; Authors - Shuai Peng
human	IHC	Oral Dis; Influence of PD-1/PD-L1 on immune micro-environment in oral leukoplakia and oral squamous cell carcinomas; Authors - Qiong Wang