Anticorps Polyclonal de lapin anti-FOXP1

FOXP1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 22051-1-AP

Synonymes

Mac-1-regulated forkhead, HSPC215, hFKH1B, Forkhead box protein P1, forkhead box P1

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Galerie de données de validation

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  WB analysis of Jurkat using 22051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22051-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22051-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22051-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 22051-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22051-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 22051-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 22051-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 22051-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22051-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22051-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 22051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 22051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 22051-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP1 (22051-1-AP) and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules LNCaP, cellules MCF-7, cellules MDA-MB-453s, cellules PC-3, cellules Raji, cellules Y79, tissu cardiaque de souris, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu embryonnaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

22051-1-AP cible FOXP1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXP1 Protéine recombinante Ag17045
• Nom complet: forkhead box P1
• Masse moléculaire calculée: 677 aa, 75 kDa
• Poids moléculaire observé: 50 kDa, 60-65 kDa, 85 kDa
• Numéro d’acquisition GenBank: BC131720
• Symbole du gène: FOXP1
• Identification du gène (NCBI): 27086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; LINC01116 promotes proliferation and migration of endometrial stromal cells by targeting FOXP1 via sponging miR-9-5p in endometriosis.; Authors - Liangyi Cui

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011