Anticorps Polyclonal de lapin anti-FOXO6

• Phare
• Validé par KD/KO

FOXO6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 19122-1-AP

Synonymes

forkhead box protein O6, FOXO6

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Galerie de données de validation

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  WB analysis of mouse brain using 19122-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19122-1-AP

  IP result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 19122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 19122-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C2C12, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

19122-1-AP cible FOXO6 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: forkhead box protein O6
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: AI361654
• Symbole du gène: FOXO6
• Identification du gène (NCBI): 100132074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has a isoform of 70 kDa when modified after translation (PMID:23639108).

Publications

Species	Application	Title
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	WB, IF	Elife; Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways.; Authors - S Michael Rothenberg ; KD Validated
pig	WB,ChIP,CoIP	RNA Biol; Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.; Authors - Xin Zong ; KD Validated
human	WB	Oncotarget; Involvement of the FOXO6 transcriptional factor in breast carcinogenesis.; Authors - François Lallemand ; KD Validated
human	IHC	Oncotarget; Elevated FOXO6 expression correlates with progression and prognosis in gastric cancer.; Authors - Jia-Hong Wang
human	IF	Mol Cell Biol; Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-dependent Manner.; Authors - Megumi Ikeda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Young-Wook (Verified Customer) (10-31-2017)

This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: 293T overexpression

FOXO6 Antibody Western Blot validation (2000 dilution) in 293T overexpression (Cat no:19122-1-AP)