Anticorps Polyclonal de lapin anti-FOLR1

• Phare
• Validé par KD/KO

FOLR1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 23355-1-AP

Synonymes

Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18

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Galerie de données de validation

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  WB analysis of HeLa using 23355-1-AP

  WB result of FOLR1 antibody (23355-1-AP, 1:1000) with si-Control and si-FOLR1 transfected HeLa cells..

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  WB analysis using 23355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23355-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 23355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 23355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 23355-1-AP

  Immunofluorescent analysis of HeLa cells using 23355-1-AP (FOLR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules JAR
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

23355-1-AP cible FOLR1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOLR1 Protéine recombinante Ag19959
• Nom complet: folate receptor 1 (adult)
• Masse moléculaire calculée: 257 aa, 30 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC002947
• Symbole du gène: FOLR1
• Identification du gène (NCBI): 2348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

Publications

Species	Application	Title
human	WB,IHC	ACS Nano; Synchronization of Nanoparticle Sensitization and Radiosensitizing Chemotherapy through Cell Cycle Arrest Achieving Ultralow X-ray Dose Delivery to Pancreatic Tumors.; Authors - Divinah Manoharan
human	WB	J Am Chem Soc; Cancer Selective Target Degradation by Folate-Caged PROTACs.; Authors - Jing Liu ; KD Validated
human	WB	ACS Appl Mater Interfaces; Design and Fabrication of Multifunctional Sericin Nanoparticles for Tumor Targeting and pH-Responsive Subcellular Delivery of Cancer Chemotherapy Drugs.; Authors - Lei Huang
human	WB	J Med Chem; Folate-Guided Protein Degradation by Immunomodulatory Imide Drug-Based Molecular Glues and Proteolysis Targeting Chimeras.; Authors - He Chen ; KD Validated
human	WB	BMC Cancer; Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1; Authors - Jiali Zhao
human	WB	Nanotechnology; PMID: 28767041; Authors - Yuwei Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marvin (Verified Customer) (12-01-2022)

The antibody had non-specific staining of the cytosol of MDA-MB-231 cells while performing immunofluorescent analysis.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Monolayer of MDA-MB-231 cells

FOLR1 Antibody Western Blot,Immunofluorescence validation (1:50 dilution) in Monolayer of MDA-MB-231 cells (Cat no:23355-1-AP)

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