Anticorps Polyclonal de lapin anti-PSMA/GCPII

PSMA/GCPII Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13163-1-AP

Synonymes

FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII

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Galerie de données de validation

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  WB analysis using 13163-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 13163-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13163-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13163-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13163-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of LNCaP using 13163-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using PSMA/GCPII antibody (13163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules PC-3, tissu hépatique humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

13163-1-AP cible PSMA/GCPII dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA/GCPII Protéine recombinante Ag3812
• Nom complet: folate hydrolase (prostate-specific membrane antigen) 1
• Masse moléculaire calculée: 719 aa, 81 kDa
• Poids moléculaire observé: 100 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC025672
• Symbole du gène: PSMA
• Identification du gène (NCBI): 2346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao
	IHC	Eur J Nucl Med Mol Imaging; Prostate Specifc Membrane Antigen in Human and Mouse Tissue; Authors -
mouse	IHC	J Nucl Med; Molecular imaging of Neuroendocrine Prostate Cancer by targeting Delta-like Ligand 3.; Authors - Joshua Aaron Korsen
human	WB	J Control Release; PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.; Authors - Jiang Ni
rat	WB	Front Pharmacol; Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress.; Authors - Kaiyun Yao
human	IHC	Appl Radiat Isot; Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers; Authors - Eva-Maria Klebermass